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PIPES, ultrapure

CAS Nr.: 5625-37-6
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Product information "PIPES, ultrapure"

Buffering substance in the pH range 6,1-7,5. Min. 99.5% pure. Good N.E. et al. (1966) Biochemistry 5, 467-77, Shipman jr. Ch. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-28.

Specificatins:
Purity: min. 99.5%
Useful pH range: pH6.1-7.5
pKa (20°C): 6.8
Water: max. 0.5%
Heavy metals (as Pb): max. 0.00001%
C8H18N2O6S2
MW = 302.4 g/mol

Classification:

CAS no.: 5625-37-6
eclass no.: 32-16-04-90
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CHAPS buffer grade

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).

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