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Product information "n-Octyl-ß-D-glucopyranoside (min. 99.0% TLC)"
Octyl glucoside (n-octyl-β-D-glucoside) is a detergent frequently used to solubilise integral membrane proteins for studies in biochemistry. It has become one of the most important detergents for purification of membrane proteins because it generally does not denature the protein and can readily be removed from final protein extracts. Above its critical micelle concentration of 0.7%, it was noted as the best detergent for improving selectivity of immunoprecipitation of phosphotyrosine modified proteins. This detergent has also been shown to rapidly inactivate infective HIV at concentrations above its CMC (about 0.7% (w/v)).
Specifications:
Assay: min. 99% (HPLC) - one spot (TLC)
Appearance: White solid crystals
[alpha]D (c 5; H2O)
: -34° +/-0.5
Absorbance (1%, H2O, 260 nm): <0.03
Solubility: >1g in 10 mL H2O or EtOH - clear colourless solution
pH (1g in 100mL): 5.5 - 7.0
Water (K.F.): max. 0.2%
Contaminations: n-Octanol: max. 0.01% (HPLC)
Contaminations: Octyl-a-glycoside: max. 0.01% (HPLC, TLC)
Chemical formula:
C14H28O6
MW = 292.38 g/mol
Application:
Component of lysis buffer to prepare mitochondrial extracts. Substance used in lysis and solubilization solutions for bacterial lipoprotein preparation.Source:
syntheticClassification:
Documents:
CertificateCategory List
Accessory Items
Highly pure Dodecyl-ß-maltopyranoside (DDM). Detergent to solubilize membrane assoziated proteins. n-Dodecyl-β-D-maltoside (DDM), a water-soluble nonionic detergent, for isolation and stabilization of hydrophobic membrane proteins. n-Dodecyl-β-D-maltoside is a gentle detergent that preserves protein activity better than many other detergents, such as CHAPS or octyl-β-glucoside.