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Product information "NADH, Nicotinamide adenine dinucleotide reduced form, disodium salt"
Synonym: β-DPNH; DPNH; Diphosphopyridine nucleotide, reduced form; beta-NADH disodium salt hydrate, reduced.
Substrate for the reduction of oxaloacetate and for the reduction of pyruvate
Composition of the buffer for the reduction of oxaloacetate: 30mM potassium phosphate pH7.5, 0.5mM oxaloacetate, 0.1mM NADH, 20mM KCl, 5mM MgCl2.
Composition of the buffer for the reduction of pyruvate: 10 mM MOPS pH6.1, 10mM Na-pyruvate, 100mM Na2CO3, 0.1mM NADH, 5mM MgCl2.
Also available from Genaxxon: M6058 NADPH tetrasodium salt, min. 98% >.
Specifications:
Assay (enzymatic): min. 95.0%
Appearance: White to light-yellow crystalline powder
Water (K.F.): max. 8.0%
Chemical formula: C21H27N7Na2O14P2
MW = 709.41 g/mol (anhydrous)
A 250nm/A 260nm (pH10): 0.82 +/- 0.03
A280nm/A260nm (pH10): 0.23 +/- 0.02
Classification:
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Accessory Items
Nicotinamide adenine dinculeotide, min. 95% (HPLC). Suitable for cell culture.Synonym: β-DPN; β-NAD, oxidised form; Coenzyme 1; Cozymase; DPN; Diphosphopyridine nucleotide; Nadide; b-Nicotinamide adenine dinucleotide; b-NAD. Used, among other things, for oxidation of malate. Composition of the measuring buffer for the oxidation of malate: 100mM Tris HCl (pH8), 10mM L-Malat, 0,5mM NAD, 20mM KCl and 1mM MnCl2.
Adenosine triphosphate (ATP) is the universal and immediately available energy source in cells and an important regulator of energy providing processes. The molecule adenosine triphosphate consists of an adenine, the sugar ribose and three phosphate (α to γ) in ester (α) or anhydride bond (β and γ). It was shown that ATP has an inhibitory effect on the inflammatory response by abrogating secretion of TNF-α and promoting secretion of Interleukin-10. Synonyms: ATP, Adenosin-5'-Triphosphat. Also available from Genaxxon: NADH Na salt (reduced) >.
Nicotinamide adenine dinucleotide phosphate reduced - Tetrasodium salt in short NADPH is the reduced version of NADP+ and can transfer a hydride atom to another reaction partner in a redox reaction. The abbreviations NADP+ for the oxidized form, NADPH for the reduced form and NADP in general are proposed by IUPAC. β-Nicotinamide adenine dinucleotide 2'-phosphate (NADP +) and β-nicotinamide adenine dinucleotide 2'-phosphate, reduced (NADPH), comprise a coenzyme redox couple (NADP+ : NADPH) involved in a variety of enzyme-catalyzed oxidation-reduction reactions. The NADP+ / NADPH redox couple facilitates electron transfer in anabolic reactions such as lipid and cholesterol biosynthesis and fatty acyl chain extension. The NADP+ / NADPH redox system is used in a variety of antioxidant mechanisms to prevent the accumulation of reactive oxidation products. NADPH is generated in vivo by the pentose phosphate pathway (PPP).