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Product information "Spermidine trihydrochloride (99%-101%)"
Spermidine inhibits neuronal nitric oxide synthase (nNOS). As it bind to DNA it might be used as precipitation agent for DNA-binding proteins. Spermidine stimulates T4 polynucleotide kinase activity.
As Spermidine and Spermidine hydrochloride deaminates over time, solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. For the same reason prepare new solutions frequently.
Specifications:
Assay (titr.): 99% - 101%
Appearance: white to almost white crystalline powder
Water: <1%
C7H19N3 x 3HCl
MW = 254.63 g/mol
Safety Information:
Classification:
Documents:
CertificateAccessory Items
DNA extracted from salmon testes. To prevent unspecific binding of a DNA to the membrane in Southern- and Northern-Hybridisations salmon sperm DNA is added to the pre-hybridisation solution (Blotto, Denhardt's etc.). This Salmon Sperm DNA shows a low molecular weight. The lyophilised DNA can be dissolved in sterile bidistilled water (stock solution e. g. 10mg/mL) and stored at -20°C. This DNA is used with a final concentration of 100μg/mL (Ref.: Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY (1989), 9.48-9.49, 13.15. Silhavy, T., et al., Experiments with Gene Fusions, Cold Spring Harbor, NY (1984), 195.)