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Product information "Hygromycin B solution - 50mg/mL"
Aminoglycoside antibiotic that inhibits growth of procaryotic micro-organisms (bacteria), eukaryotic micro-organisms (yeast) and mammalian cells. Inhibits protein synthesis at translational step: causes misreading of mRNA. A gene from E.coli encoding resistance to hygromycin B can be isolated and cloned by recombinant DNA technology. It is useful for the identification or selection of recombinant clones in various cell types. The working concentration for mammalian cell selection is normally between 50µg/mL and 1mg/mL, plant cells: 20µg/mL-200µg/mL, bacteria: 20µg/mL-200µg/mL and Fungi: 200µg/mL - 1mg/mL. The appropriate concentration should be tested experimentally.
Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics > as powder or solutions, e.g., Geneticin G418 > or Kanamycin sulfate >.
Specifications:
50 mg/mL sterile filetered solution
Heavy metals: <20ppm
Endotoxin tested (<10EU/mL)
C20H37N3O13
MW = 527.53 g/mol
Toxic/harmful: may be cancerogenic or teratogenic.
Working solutions (<2mg/mL) are stable for about 1 month at +2°C to +8°C. It is recommended to use end concentrations between 100µg/mL and 800µg/mL.
Safety Information:
Classification:
Documents:
Safety Data SheetCertificate
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G418 also known Geneticin(TM) is an aminoglycoside antibiotic that inhibits protein synthesis in mammalian cells by interfering with ribosomal function. Geneticin shows a structure similar to gentamycin > and kanamycin >, and finds application in the selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601. Because G418 is toxic for many cells, the optimum concentration for each cell type has to be determined. It is usually between 50ug/mL and 1000μg/mL. At a concentration of 500μg/mL, for example, 70% of HepG2 cells are killed after incubation for one week and 100% will stop their growth.Since potency of the antibiotic can vary from batch to batch, it is recommended to create large stocks of a batch tested to standardize the system. Cells divide still once or twice in the presence of the lethal dose of G418, so that a selection effect will be visible only after several days. Geneticin is a registered trademarks of Gibco a Division of Life Technologies. Genaxxon's G418 is an alternative to ThermoFisher / Invitrogen product no. 10131035 and 10131027, or a replacement for BioChrom product no.: A 2812.
Gentamycin sulfate is an aminoglycoside antibiotic and a metabolic product of Micromonospora purpurea. It is a mixture of 3 closely related components: C1 (approx. 20-40 %), C2 (approx. 40-60 %), and C1a (approx. 10-30 %) (Cox d. et al. (1977) Sammes, P.G (ed.) Topics in antibiotics chemistry Vol. I, Chichester, Horwood, pp. 1-190, Lancini G., Parenti F. (1982) Antibiotics: Springer, New York). The chemical formula and mol weights are: C19-21H39-43N5O7x2,5 H2SO4 with mol weights of 694.75 up to 723.75 g/mol. Gentamycin is active against Gram-positive and Gram-negative bacteria and Mycoplasma! Mechanism: Inhibits protein biosynthesis by binding to the 30 S ribosomal subunit and by destroying the cell membrane. The chemical formula and mol weights are: C19-21H39-43N5O7x2,5 H2SO4 with mol weights of 694.75 up to 723.75 g/mol. Gentamycin is active against Gram-positive and Gram-negative bacteria and Mycoplasma! Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics > as powder or solutions, e.g., Geneticin G418 > or Kanamycin sulfate >.