G418 Powder (min. 98% DC)

G418 also known Geneticin(TM) is an aminoglycoside antibiotic that inhibits protein synthesis in mammalian cells by interfering with ribosomal function. Geneticin shows a structure similar to gentamycin > and kanamycin >, and finds application in the selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601. Because G418 is toxic for many cells, the optimum concentration for each cell type has to be determined. It is usually between 50ug/mL and 1000μg/mL. At a concentration of 500μg/mL, for example, 70% of HepG2 cells are killed after incubation for one week and 100% will stop their growth.Since potency of the antibiotic can vary from batch to batch, it is recommended to create large stocks of a batch tested to standardize the system. Cells divide still once or twice in the presence of the lethal dose of G418, so that a selection effect will be visible only after several days. Geneticin is a registered trademarks of Gibco a Division of Life Technologies. Genaxxon's G418 is an alternative to ThermoFisher / Invitrogen product no. 10131035 and 10131027, or a replacement for BioChrom product no.: A 2812.

Aminoglycoside antibiotic that inhibits growth of procaryotic micro-organisms (bacteria), eukaryotic micro-organisms (yeast) and mammalian cells. Inhibits protein synthesis at translational step: causes misreading of mRNA. A gene from E.coli encoding resistance to hygromycin B can be isolated and cloned by recombinant DNA technology. It is useful for the identification or selection of recombinant clones in various cell types. The working concentration for mammalian cell selection is normally between 50µg/mL and 1mg/mL, plant cells: 20µg/mL-200µg/mL, bacteria: 20µg/mL-200µg/mL and Fungi: 200µg/mL - 1mg/mL. The appropriate concentration should be tested experimentally. Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics > as powder or solutions, e.g., Geneticin G418 > or Kanamycin sulfate >.

Kanamycin is very stable as a solution in the pH range 6-8 and can be stored for 6-8 months at room temperature (with only 1-2% loss of activity). Autoclaving at 120°C (even for more than 1 hour) leads to only a slight loss of activity (5%). When exposed to air, Kanamycin may change colour but without losing much of its activity. The change in colour can be prevented by antioxidants. Aminoglycoside-antibiotic with broad antibacterial spectrum, (including mycobacteria, many gram-positive and most gram-negative organisms), Additive in culture media for the isolation of group D Streptococci on Kanamycin Esculin Azide Agar (Mossel et al.) or for selection of transformed plant cells containing the neomycin phosphotransferase (for kanamycin resistance), on a kanamycin-medium. Inhibits formation of peptide bonds, Cox d. et al. (1977) Sammes, P.G (ed.) Topics in antibiotics chemistry Vol. I, Chichester, Horwood, pp. 1-190, Lancini G., Parenti F. (1982) Antibiotics: Springer, New York, Pestka S. (1971) Annu. Rev. Microbiol. 25, 487-562.

Gentamycin sulfate is an aminoglycoside antibiotic and a metabolic product of Micromonospora purpurea. It is a mixture of 3 closely related components: C1 (approx. 20-40 %), C2 (approx. 40-60 %), and C1a (approx. 10-30 %) (Cox d. et al. (1977) Sammes, P.G (ed.) Topics in antibiotics chemistry Vol. I, Chichester, Horwood, pp. 1-190, Lancini G., Parenti F. (1982) Antibiotics: Springer, New York). The chemical formula and mol weights are: C19-21H39-43N5O7x2,5 H2SO4 with mol weights of 694.75 up to 723.75 g/mol. Gentamycin is active against Gram-positive and Gram-negative bacteria and Mycoplasma! Mechanism: Inhibits protein biosynthesis by binding to the 30 S ribosomal subunit and by destroying the cell membrane. The chemical formula and mol weights are: C19-21H39-43N5O7x2,5 H2SO4 with mol weights of 694.75 up to 723.75 g/mol. Gentamycin is active against Gram-positive and Gram-negative bacteria and Mycoplasma! Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics > as powder or solutions, e.g., Geneticin G418 > or Kanamycin sulfate >.

Aminoglycoside-antibiotic with broad antibacterial spectrum, (including mycobacteria, many gram-positive and most gram-negative organisms), Additive in culture media for the isolation of group D Streptococci on Kanamycin Esculin Azide Agar (Mossel et al.) or for selection of transformed plant cells containing the neomycin phosphotransferase (for kanamycin resistance), on a kanamycin-medium. Inhibits formation of peptide bonds, Cox d. et al. (1977) Sammes, P.G (ed.) Topics in antibiotics chemistry Vol. I, Chichester, Horwood, pp. 1-190, Lancini G., Parenti F. (1982) Antibiotics: Springer, New York, Pestka S. (1971) Annu. Rev. Microbiol. 25, 487-562.