CMRL-1066 without L-Glutamine, without Phenol red

Pen-Strep: Penicillin G from Penicillium notatum and Streptomycin from Streptomyces griseus prevent together against gram-positive and gram-negative bacteria the bacterial contamination of cell cultures. Penicillin G acts by interfering directly with turnover of bacterial cell wall and indirectly by triggering release of enzymes that alter the cell wall. Streptomycin works by attaching to the 30S subunit of the bacterial ribosome, which leads to an inhibition of protein synthesis and the subsequent death of bacteria.Penicillin G potassium salt is soluble in water. At +4°C or room temperature, the dry substance is stable for approximately 5 years. The buffered solution (pH4,5-7) will loose 5% activity after 20 days at +4°C respective 20% after 60 days at +4°C. At room temperature, reduction in activity will be 5% after only 48 hours, 10% after 7 days, and 20% to 50% after 14 days. In an unbuffered solution stability is considerably lower (about 3-7 days at +4°C). Pen-Strep: Penicillin G and Streptomycin against bacterial contamination of cell cultures. Genaxxon bioscience offers besides Pen-Strep a broad range of Antibiotics and Antimycotics as powder or solutions, e.g., Geneticin G418 or Gentamycin sulfate.The minimum order quantity is 5 × M3140.0100 (100 mL).

G418 also known Geneticin(TM) is an aminoglycoside antibiotic that inhibits protein synthesis in mammalian cells by interfering with ribosomal function. Geneticin shows a structure similar to gentamycin > and kanamycin >, and finds application in the selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601. Because G418 is toxic for many cells, the optimum concentration for each cell type has to be determined. It is usually between 50ug/mL and 1000μg/mL. At a concentration of 500μg/mL, for example, 70% of HepG2 cells are killed after incubation for one week and 100% will stop their growth.Since potency of the antibiotic can vary from batch to batch, it is recommended to create large stocks of a batch tested to standardize the system. Cells divide still once or twice in the presence of the lethal dose of G418, so that a selection effect will be visible only after several days. Geneticin is a registered trademarks of Gibco a Division of Life Technologies. Genaxxon's G418 is an alternative to ThermoFisher / Invitrogen product no. 10131035 and 10131027, or a replacement for BioChrom product no.: A 2812.