5/6-JOE mixed isomer

5/6-TAMRA is a mixtures of the 5' and 6' isomers of carboxytetramethylrhodamine (TMR) free acid. The non-activated single isomer carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543nm spectral line of the green He-Ne laser. Succinimidyl esters of 5-TAMRA >, 6-TAMRA > or of the mixed 5/6 TAMRA isomers > are the labeling reagents of choice for the preparation of fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially for fluorescent antibodies or avidine derivatives used in immunochemistry. These fluorescent dyes are also used as acceptors for FAM > fluorophores in FRET studies. More Fluorescent Dyes from Genaxxon: 5/6-TAMRA >, 5-TAMRA >, FAM >, ROX >, DAF-2 >.

6-TAMRA is the pure 6-isomer of carboxytetramethylrhodamine (TMR) free acid. The non-activated single isomer carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543nm spectral line of the green He-Ne laser. Succinimidyl esters of 5-TAMRA >, 6-TAMRA > or of the mixed 5/6 TAMRA isomers > are the labeling reagents of choice for the preparation of fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially for fluorescent antibodies or avidine derivatives used in immunochemistry. These fluorescent dyes are also used as acceptors for FAM > fluorophores in FRET studies. More Fluorescent Dyes from Genaxxon: 5/6-TAMRA >, 5-TAMRA >, FAM >, ROX >, DAF-2 >.

The single isomer 6-FAM (6-Carboxyfluorescein) is an amine-reactive derivative of Fluorescein giving stable derivatives upon conjugation with biopolymers. The single amine-reactive fluorescein derivatives 5-FAM > and 6-FAM > and the mixed isomers form 5/6-FAM > are the most common fluorescent derivatizing reagents for the covalently labeling of proteins. Carboxyfluorescein can be retained much better in cells than fluorescein since its pKa is lower than the cytosolic pH of most cells. 5-FAM, 6-FAM and 5/6-FAM contain a carboxylic acid which can react with primary amines via carbodiimide activation. Carboxyfluorescein is commonly used as a polar tracer.Other fluorescent dyes from Genaxxon: TAMRA >, ROX >, DAF-2 >

6-JOE (6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein) and other fluoreszence dyes. Genaxxon bioscience offers a broad range of different fluorscence dyes for labelling probes or for other research purposes. This non-activated fluorescein derivative is used as an orange fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. Genaxxon bioscience offers a wide range of fluorescent dyes for designing your probe or for other research purposes. More fluorescent dyes > from Genaxxon: S5409 FAM >, S5410 TAMRA >, S5439 DAF-2 >, etc.

5-TAMRA is the pure 5-isomer of carboxytetramethylrhodamine (TMR) free acid. The non-activated single isomer carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543nm spectral line of the green He-Ne laser. Succinimidyl esters of 5-TAMRA >, 6-TAMRA > or of the mixed 5/6 TAMRA isomers > are the labeling reagents of choice for the preparation of fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially for fluorescent antibodies or avidine derivatives used in immunochemistry. These fluorescent dyes are also used as acceptors for FAM > fluorophores in FRET studies. More Fluorescent Dyes from Genaxxon: 5/6-TAMRA >, 5-TAMRA >, FAM >, ROX >, DAF-2 >.

The mixed isomers 5/6-FAM (5/6-Carboxyfluorescein) is an amine-reactive derivative of Fluorescein > giving stable derivatives upon conjµgation with biopolymers. The amine-reactive fluorescein derivatives 5-FAM > and 6-FAM > and the mixed isomers form 5/6-FAM > are the most common fluorescent derivatizing reagents for the covalently labeling of proteins. Carboxyfluorescein can be retained much better in cells than fluorescein since its pKa is lower than the cytosolic pH of most cells. 6-FAM contains a carboxylic acid which can react with primary amines via carbodiimide activation. Carboxyfluorescein is commonly used as a polar tracer.Other fluorescent dyes from Genaxxon: TAMRA >, ROX >, DAF-2 >

5-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein and other fluoreszence dyes. Genaxxon bioscience offers a broad range of different fluorscence dyes for labelling probes or for other research purposes. This non-activated fluorescein derivative is used as an orange fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. Genaxxon bioscience offers a wide range of fluorescent dyes for designing your probe or for other research purposes. More fluorescent dyes > from Genaxxon: S5409 FAM >, S5410 TAMRA >, S5439 DAF-2 >, etc.