6-TAMRA, single isomer

5/6-TAMRA is a mixtures of the 5' and 6' isomers of carboxytetramethylrhodamine (TMR) free acid. The non-activated single isomer carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543nm spectral line of the green He-Ne laser. Succinimidyl esters of 5-TAMRA >, 6-TAMRA > or of the mixed 5/6 TAMRA isomers > are the labeling reagents of choice for the preparation of fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially for fluorescent antibodies or avidine derivatives used in immunochemistry. These fluorescent dyes are also used as acceptors for FAM > fluorophores in FRET studies. More Fluorescent Dyes from Genaxxon: 5/6-TAMRA >, 5-TAMRA >, FAM >, ROX >, DAF-2 >.

6-ROX (6-carboxy-X-rhodamine) is a derivative of the amine-reactive carboxy-X-rhodamine (ROX). Compared to the fluoresceine family of dyes, Rhodamine dyes are more resistant to photo bleaching and stable over a wider pH range. The relatively long wavelength emission maximum for ROX makes it an attractive choice in multiplex detection systems. 5-ROX >, 6-ROX > and their mixture 5/6-ROX > are used in the labeling of biomolecules by EDC-mediated reactions. Derivatives of ROX are used in the labeling of proteins, peptides and other biological ligands. Succinimidyl ester with the derivative 5/6-carboxy-X-rhodamine (5(6) Carboxy-X-rhodaminsuccinimidylester / 5(6) ROX SE >) are frequently used in the oligonucleotide labeling and for automated applications in DNA sequencing. Genaxxon bioscience offers a wide range of fluorescent dyes for designing your probe or for other research purposes. More fluorescent dyes > from Genaxxon: S5409 FAM >, S5410 TAMRA >, S5439 DAF-2 >, etc.

DAF-2 (4,5-Diaminofluorescein) shows higher photostability than the classical fluorescein derivative DAF. Applications: DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxideproducing neurons in brain slices, in mitochondria and in living plant cells. In the cell, the product reacts rapidly with NO in the presence of O2 to form the highly fluorescent compound triazolofluorescein (DAF-2T (Cat-No. S5442 >)). References: (1) M. Kirsch et al.; J. Pineal Res. 40(1), 10 (2006), (2) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003), (3) N. Nagata et al.; J. Biochem. 125(4), 658 (1999), (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al.; Anal. Chem. 70, 2446 (1998)

Amine-reactive carboxytetramethylrhodamine is one of the most commonly used red fluorescent dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm). This single isomer amine-reactive red fluorescent derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.

5-TAMRA is the pure 5-isomer of carboxytetramethylrhodamine (TMR) free acid. The non-activated single isomer carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543nm spectral line of the green He-Ne laser. Succinimidyl esters of 5-TAMRA >, 6-TAMRA > or of the mixed 5/6 TAMRA isomers > are the labeling reagents of choice for the preparation of fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially for fluorescent antibodies or avidine derivatives used in immunochemistry. These fluorescent dyes are also used as acceptors for FAM > fluorophores in FRET studies. More Fluorescent Dyes from Genaxxon: 5/6-TAMRA >, 5-TAMRA >, FAM >, ROX >, DAF-2 >.

5/6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein and other fluoreszence dyes. Genaxxon bioscience offers a broad range of different fluorscence dyes for labelling probes or for other research purposes. This non-activated fluorescein derivative is used as an orange fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. Genaxxon bioscience offers a wide range of fluorescent dyes for designing your probe or for other research purposes. More fluorescent dyes > from Genaxxon: S5409 FAM >, S5410 TAMRA >, S5439 DAF-2 >, etc.

5(6)-Carboxytetramethylrhodamine succinimidyl ester (5(6) TAMRA-SE) is an amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm). This amine-reactive red fluorescent derivatization reagent is widely used for covalent labeling of proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.

Amine-reactive carboxytetramethylrhodamine is one of the most commonly used red fluorescent dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm). This single isomer amine-reactive red fluorescent derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.