Advantages at a glance
- Purity: min. 99.5% (titr.)
- Pyrogen test: <20EU/g
- Density (d20°/4°): 1.100
- Free acid: max: 0.001%
- Non-volatile matter: max. 0.001%
- Water (K.F.): max. 0.1%
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Product information "DMSO, Cell culture grade"
DMSO is an aprotic polar solvent used in chemical reactions, in PCR and as a cryoprotectant agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It can be used for the deep-freeze storage of primary, subcultivated and recombinant heteroploid and hybridoma cell lines as well as embryonic or hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).
Dimethyl sulfoxide (DMSO) is used for the production of fresh, competent bacterial cells and for the long-term storage of bacteriophage l (final concentration 7%).
Since the oxidation products of DMSO inhibit the transformation, fresh DMSO should be aliquoted and stored frozen at -20°C.
Specifications:
Purity: min. 99.5% (titr.)
Pyrogen test: <20EU/g
Density (d20°/4°): 1.100
Free acid: max: 0.001%
Non-volatile matter: max. 0.001%
Water (K.F.): max. 0.1%
MP = 18°C
BP = 189°C
C2H6OS
MW = 78.1 g/mol
Application:
Endotoxin tested for used in cell culture applications.Classification:
Documents:
Safety Data SheetCertificate
Source: NCBI PubMed
DMSO
DMSO is an aprotic polar solvent used in chemical reactions, in PCR and as a cryoprotectant agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It can be used for the deep-freeze storage of primary, subcultivated and recombinant heteroploid and hybridoma cell lines as well as embryonic or hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). Dimethyl sulfoxide (DMSO) is used for the production of fresh, competent bacterial cells and for the long-term storage of bacteriophage l (final concentration 7%). Since the oxidation products of DMSO inhibit the transformation, fresh DMSO should be aliquoted and stored frozen at -20°C.
DMSO is an aprotic polar solvent that is used in chemical reactions as well as in PCR or as anti-freeze protection in the deep-freeze storage of cells, tissues and organs (sterile DMSO only). By adding DMSO for annealing before the sequencing reaction and adding 10% DMSO (final concentration) for the labeling reaction and for termination, the quality of the sequencing of Miniprep DNA can be increased. The amplification of RNA by RT-PCR is also improved by adding DMSO. DMSO is used during reverse transcription (RT) and PCR. The DMSO concentration depends on the RNA species.
Dimethyl sulfoxide. Highly active solvent and pharmaceutical vehicle, for freezing cells. For gradient centrifugation. Determination of cysteine and cystine in proteins.