Advantages at a glance
- High Gel Strength Agarose for PFGE and Blotting
- Gel strength (1%): >1800 g/cm2
- Gel strength (1.5%): >3200 g/cm2
- Gelling temperature: 36°C (± 1.5°C)
- Melting temperature: >88°C (± 1.0°C)
- Nuclease activity: not detected
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Product information "Agarose Mega"
Agarose Mega resolved DNA and RNA fragments from >100bp up to 50kb. Due to the high gel strength the agarose is ideally suited for Southern- and Northern blotting experiments or Pulsed Field Gel Electrophoresis (PFGE) as gel will not break during handling or electrophoresis. Due to the high gel strength, low concentrations of agarose may be used and high molecular weight molecules migrate rapidly without significant resctriction. The high gel strength reduces separation times for large DNA molecules while maintaining high resolution. Agarose Mega can also be used for the preparative separation of nucleic acids or for Ouchterlony or radial immundiffusion experiments.
Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.
Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use.
Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.
High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).
For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
Specifications for A mega:
Electroendosmosis (EEO): <0.12
Gel strength (1%): >1800 g/cm2
Gel strength (1.5%): >3200 g/cm2
Gelling temperature: 36°C (± 1.5°C)
Melting temperature: >88°C (± 1.0°C)
pH (1% in water): 6.0
Loss on drying: max. 7%
Sulfate: <0.15%
Ash: max. 0.25%
DNAse and RNAse: not detected
Application:
for PFGE electrophoresis (pulsed field gel electrophoresis)Source:
seaweedClassification:
Documents:
Safety Data SheetCertificate
General Data 1
General Data 2
Agaroses – A Solution for Every Application from GENAXXON!
Accessory Items
The High-Fidelity Pwo proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pwo DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus woesei. Pwo proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase. A mixture of Taq DNA Polymerase and Pwo DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermo stability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:- M3003 ReproFast Proofreading Polymerase- M3004 Pfu Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase - AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.
Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker. The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL). The recommended amount of DNA marker per lane is 0,5μg (5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of up to >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer contains 15mM MgCl2. Free Taq DNA Polymerase test sample available!No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-E'. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer contains 25mM MgCl2. Taq DNA Polymerase test sample available! No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.