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RealQ Plus 2x Master Mix Green - Low ROX

Product information "RealQ Plus 2x Master Mix Green - Low ROX"

Introduction:

Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular beacons) and DNA-binding fluorescent dyes (e.g. ethidium bromide, SYBR® Green, EvaGreen®, PicoGreen®). Ampliqon offers the RealQ Plus 2x Master Mix in two formulations: for probe or with DNA-binding fluorescent dye, making them ideal for most quantitative PCR applications.

The RealQ Plus 2x Master Mixes are available with high, low or without ROX™ for optimal performance on most of the commonly used real-time PCR instruments. The RealQ Plus 2x Master Mixes promote high specificity and low background by using TEMPase Hot Start DNA Polymerase, a modified Taq DNA polymerase with hot start capabilities.

The RealQ Plus 2x Master Mix Green with low ROX™ is a singletube 2x reagent including all components necessary to perform real-time DNA amplification for DNA-binding dye based PCR. Just add your primers and DNA. The ROX™ internal reference dye level is optimized for real-time instruments that require low ROX™ as internal reference dye e.g. the Stratagene Mx3005P.

Detection limit: approximately 1 copy.

Quantitation limit: approximately 24 copies (0.08ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copies per diploid genome)

ompatibility: Real-time instruments which require low ROX™ as internal reference dye e.g. the Stratagene Mx3005P.

Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

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Regular price: €115.00