InhibiCore Rapid Plant DNA Q-Extraction PCR Kit

Direct PCR from Plant Samples – Fast, Robust and Inhibitor-Resistant

The InhibiCore Rapid Plant DNA Q-Extraction PCR Kit is a streamlined, high-performance solution for rapid DNA analysis from plant material. It combines the efficient Plant DNA Q-Extraction Solution (M3230) with the highly robust InhibiCore Rapid Plant PCR MasterMix (5X) (M3250), enabling a complete workflow from sample preparation to reliable qPCR analysis.

Plant tissues often contain strong PCR inhibitors such as polyphenols, polysaccharides, and other secondary metabolites. This kit is specifically designed to overcome these challenges, delivering reproducible and highly sensitive qPCR results even from difficult plant matrices.

The Plant DNA Q-Extraction Solution enables fast and efficient DNA release without the need for conventional, time-consuming extraction procedures. Combined with the inhibitor-resistant PCR master mix, it creates a seamless Direct-PCR workflow that significantly reduces hands-on time and simplifies the overall process.

The included InhibiCore Rapid Plant PCR MasterMix (5X) is based on a modified HotStart Taq DNA polymerase, ensuring high specificity, sensitivity, and robustness. Its exceptional inhibitor tolerance makes it ideally suited for demanding applications in plant research, agriculture, and quality control. The kit is also lyo-ready and includes dUTP for optional carry-over contamination control when used with UDG (Uracil-DNA Glycosylase).

The HotStart polymerase enables convenient room-temperature setup without cooling or activation steps.

Key Benefits

• Complete solution for fast direct PCR from plant material
• Seamless workflow: DNA extraction + qPCR in one system
• High inhibitor tolerance for robust amplification from plant samples
• Fast and simple sample preparation without classical DNA isolation
• High sensitivity and specificity for reliable results

Typical Applications

• Plant genotyping 
• GMO and transgene detection 
• Agricultural and plant research 
• Plant pathogen detection 
• Seed and quality control 
• High-throughput direct PCR from plant material

Applications examples:

1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysates

Reliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) and fluorescent dye chemistry.
A serial dilution of wheat lysate (10% to 0.001%), prepared with Plant DNA Q-Extraction Solution (M3230) was used to evaluate sensitivity and inhibitor tolerance.

Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps.

Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).
The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products.

2. Benchmark of Direct PCR performance from 10% tomato lysate

Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysate
Direct PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates.