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Total Plant RNA Purification Kit

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Product information "Total Plant RNA Purification Kit"

Genaxxon´s Total Plant RNA Purification Kit is designed for the rapid and efficient purification of high quality RNA from fresh (100mg) or dryed (50mg) plant tissue samples. 

The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total Plant RNA Purification Kit utilises spin columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. 
The isolation procedure consists of 5 steps. In the first step, cells are harvested and then lysed in the second step. Any RNases are inactivated. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth. 
Genaxxon offers also ceramic beads for rapid cell disintegration and other RNA purification kits.

Specifications: 

For 100mg fresh plant tissue material
For 50mg dried plant tissue material
Yield: up to 30µg RNA

Application:

RNA purification for downstream applications as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RT-PCR, RNA sequencing, micro arrays, etc.

Classification:

eclass no.: 32170301
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Manuals