Advantages at a glance
- Fast procedure and delivering high-quality total RNA.
- Ready-to-use RNA for high performance in any downstream application.
- Consistent RNA yield from a small amount of starting material.
Product number:
GS5300.0100
Our benefits
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
For precise information regarding the delivery date, please contact Genaxxon.
Product information "Total RNA Purification Kit - Blood/Cell culture"
Genaxxon´s Total RNA Purification Kit - Whole Blood/Cell culture is designed for the rapid and efficient purification of high quality RNA from whole blood (up to 300µL) or mammalian/bacterial/fungi cells.
The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Kit utilises spin columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 5 steps. In the first step, cells are harvested and then lysed in the second step. Any RNases are inactivated. The two-step washing stage effectively removes impurities and enzyme inhibitors.
The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.
Genaxxon offers also ceramic beads for rapid cell disintegration and other RNA purification kits.
With our HotScriptase RT Mastermix, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step.
Specifications:
Binding capacity of columns: approximately 230µg RNA
For up to 300µL whole blood
For up to 1x 10E7 mammalian cells
For up to 1x 10E9 bacterial cells
For up to 1x 10E8 fungus cells
Application:
RNA purification for downstream applications as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RT-PCR, RNA sequencing, micro arrays, etc.Classification:
eclass no.:
32170301
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.