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Product information "CHES, min. 99.0%"
CHES shows a pKa (25°C) of 9,55 which makes CHES useful as a buffering component in the pH range between 8.6 to 12.0. CHES interferes in the protein quantification according to Lowry.
It is used in crystallisation of Phosphotriesterase (50mM) or in the chemical modification of Bacteriorhodopsin (10mM). Synonyms are: 2-(Cyclohexylamino)ethanesulfonic acid; 2-(N-Cyclohexylamino)ethanesulfonic acid.
Specifications:
Assay (titr.): min. 99%
Appearance: white powder
pKa 25 = 9.55
A (1cm/0.1M in water) 260 nm = 0.05; 280 nm = 0.04
pH (1%, H2O) = 5 - 6
Useful pH-range: 8.6 - 10.
C8H17NO3S
MW = 207.29 g/mol
Application:
Used in crystallisation of Phosphotriesterase (50mM) or in the chemical modification of Bacteriorhodopsin (10mM).Classification:
Documents:
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Accessory Items
MOPS is used as a buffer substance at pH 6.5 to 7.9. (Goods N.E., Izawa S. (1972) Methods Enzymol., 24, 53, Ferguson W.K. et al. (1980) Anal. Biochem., 104, 300).
3-(Cyclohexylamino)-1-propane sulfonic acid
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).