Advantages at a glance
- Purity: min. 99.5%
- pK25 = 7.2 pH (0.1M in H2O) 3.5 to 4.0
- A(1cm/0.1 M in water): max. 0.05 at 260nm, max. 0.05 at 280nm
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Product information "MOPS buffer grade, min. 99.5%"
MOPS is used as a buffer substance at pH 6.5 to 7.9. (Goods N.E., Izawa S. (1972) Methods Enzymol., 24, 53, Ferguson W.K. et al. (1980) Anal. Biochem., 104, 300).
Specifications:
Purity: min. 99.5%
pK25 = 7.2
pH (0.1M in H2O) 3.5 to 4.0
MP = 277-280°C
Fe: max. 0.0001%
Pb: max. 0.001%
A(1cm/0.1 M in water): max. 0.05 at 260nm, max. 0.05 at 280nm
C7H15NO4S
MW = 209.27 g/mol
Application:
Biological buffer for pH range 6.5 to 7.9Classification:
Documents:
CertificateCategory List
Accessory Items
CHES shows a pKa (25°C) of 9,55 which makes CHES useful as a buffering component in the pH range between 8.6 to 12.0. CHES interferes in the protein quantification according to Lowry. It is used in crystallisation of Phosphotriesterase (50mM) or in the chemical modification of Bacteriorhodopsin (10mM). Synonyms are: 2-(Cyclohexylamino)ethanesulfonic acid; 2-(N-Cyclohexylamino)ethanesulfonic acid. Other buffers are: BES, BICIN, MOPS, CAPS, CHAPS
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).