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Product information "TAPS p.A., min. 99%"
TAPS (N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid) is used in biochemistry, molecular biology, microbiology and cell culture as buffer substance with optimal buffer range at pH7.7-9.1 (pKa = 8.4 (20°C). Hepes is one of the "goods buffers." Dr. Norman Good et al. descriped in his work in 1966 a selection of "biological buffers" that display similar characteristics. These characteristics include: pKa value between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbence between 240 nm and 700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations, and limited permeability of biological membranes.
Goods buffers are: BES, BICINE, CAPS, EPPS, HEPES, MES, MOPS, PIPES, TAPS, TES, TRICINE.
Reference:
Good N.E. et al. (1966) Hydrogen Ion Buffers for Biological Research, Biochemistry 5, 467-77.
Shipman jr. Ch. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-10.
Specifications:
Assa: 99% - 101%
pK25 = 8.4
pH (0.1M in H2O) 4.5 to 6.0
Loss on drying: max. 1%
A(1cm/0.1 M in water): max. 0.02 at 260nm, max. 0.01 at 280nm
C7H17NO6S
MW = 243.28 g/mol
Application:
Biological buffer for pH range 7.7 to 9.1Source:
syntheticClassification:
Documents:
Category ListAccessory Items
CHES shows a pKa (25°C) of 9,55 which makes CHES useful as a buffering component in the pH range between 8.6 to 12.0. CHES interferes in the protein quantification according to Lowry. It is used in crystallisation of Phosphotriesterase (50mM) or in the chemical modification of Bacteriorhodopsin (10mM). Synonyms are: 2-(Cyclohexylamino)ethanesulfonic acid; 2-(N-Cyclohexylamino)ethanesulfonic acid. Other buffers are: BES, BICIN, MOPS, CAPS, CHAPS
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).