Agarose LE - Standard Agarose
Advantages at a glance
- Standard agarose
- Standard Melting Point
- Separation range: 100bp to 25kbp
- Low EEO
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€85.60*
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€59.92*
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Ready to ship today,
Delivery time 1-2 workdays
Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!
Agarose LE is a standard agarose for the separation of DNA in the size range between 100bp and 25kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc.
This agarose is comparable with, e.g. Agarose BioRagent, low EEO from Sigma or with the Universal-Agarose, peqGOLD from Peqlab. Free Taq DNA Polymerase test sample available! No shipping costs within Germany.
Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.
Standard normal melting agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 100bp up to 25kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use.
Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.
High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).
For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
Specifications:
Electroendosmosis (EEO): <0.12
Sulfate: <0.2%
Gel strength (1.0%): >1000 g/cm2
Gel strength (1.5%): >2300 g/cm2
Gelling temperature: +36°C (+/- 1.5°C)
Melting temperature: <89°C
Water content: <9%
pH-value in water: 6.0 to 7.0 (1% in water)
DNAses and RNAses: not detected.
DNA binding: not detected
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
EC-Nr: 232-731-8CAS-Nr: 9012-36-6
eclass-Nr: 32-16-04-03
Documents:
Safety Data SheetCertificate
Manuals
Spec. Product Description
General Data 1
General Data 2
Source: NCBI PubMed
Christoph Dockter, Damian Gruszka, Ilka Braumann, Arnis Druka, Ilze Druka, Jerome Franckowiak, Simon P. Gough, Anna Janeczko, Marzena Kurowska, Joakim Lundqvist, Udda Lundqvist, Marek Marzec, Izabela Matyszczak, André H. Müller, Jana Oklestkova, Burkhard Schulz, Shakhira Zakhrabekova, Mats Hansson
Plant Physiol. 2014 Dec; 166(4): 1912–1927. Published online 2014 Oct 20. doi: 10.1104/pp.114.250738
PMCID: PMC4256852
Philip Ifesinachi Anochie, Edwina Chinwe Onyeneke, Emmanuel Osaretin Asowata, Ebelechukwu Afocha, Anthony Chidiebere Onyeozirila, Angelina Chinyere Ogu, Bestman Chukwuemeka Onyeneke
Germs. 2013 Sep; 3(3): 81–89. Published online 2013 Sep 1. doi: 10.11599/germs.2013.1041
PMCID: PMC3882862
Andrea Bader, Bettina Klein, Heinz Breer, Jörg Strotmann
Front Neural Circuits. 2012; 6: 84. Prepublished online 2012 Oct 16. Published online 2012 Nov 16. doi: 10.3389/fncir.2012.00084
PMCID: PMC3499762
Brief instructions for a standard agarose gel (2%, 100mL)
- Weigh 2g agarose LE into a heat-resistant bottle.
- Prepare a 1% TAE solution in a 1L flask (e.g. 20mL 50X ready-to-use TAE buffer solution > fill up to 1000mL distilled water), stir well..
- Add 100mL of this solution to the agarose, swirl gently until the agarose has dispersed in the liquid.
- Carefully heat this agarose solution in the microwave at short intervals until the agarose has completely dissolved. A clear solution is formed.
- Caution: The solution can easily boil over during heating, always observe and choose short intervals!
- Caution: Solution becomes very hot, use gloves! Avoid boiling delay! Wear safety goggles!
- The solution should be cooled to approx. 50-60°C (glass can just be touched).
- Depending on the application, a gel dye, e.g. SafeGel red stain > (~10µL per 100mL gel, depending on the desired intensity of the bands) is added to the gel and evenly distributed.
- The solution is now carefully poured into the prepared slide (with comb) of an electrophoresis chamber.
- Caution: If the gel solution is still too hot, there is a risk that it will leak through insufficiently sealed cracks in the slide or that the slide will break! If the solution has cooled down too much, the gel will already have gelled!
- Allow the gel to solidify for approx. 1 hour.
- Add the remainder of the 1X TAE buffer to the prepared electrophoresis chamber and place the gel with slide in the appropriate position (pockets are on top). The gel must be completely covered with liquid. The comb is pulled out before electrophoresis.
- Before applying the samples, it is recommended to rinse the pockets with the running buffer to remove gel residues.
- Important: The TAE concentration of the electrophoresis buffer (running buffer) must match the concentration in the gel!
- The PCR product is mixed with loading buffer (e.g. DNA loading buffer I >) (6X loading buffer: 5µL product + 1µL loading buffer) and carefully added to the pockets.
- The step of mixing with a loading buffer is omitted when using a master mix in the PCR, in which a loading buffer is already integrated (e.g. Genaxxon's RedMasterMix 2X Taq PCR Mastermix with red dye >).
- The additional application of a DNA size marker is recommended (e.g. GenLadder 100bp + 1.5kb, ready-to-use >), approx. 5-6µL per lane.
- Depending on the device manufacturer's specifications, gel electrophoresis can now be started after the electrodes have been attached. The negatively charged DNA will migrate towards the positive pole.
Example: 120V, 160 mA, 50W.
The running time depends on the desired result (on average 0.5-1 hour). The result can be recorded with a photo documentation device.
