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2',3'- Dideoxyadenosine-5'-O-triphosphate (ddATP)

CAS Nr.: 132619-65-9
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Product information "2',3'- Dideoxyadenosine-5'-O-triphosphate (ddATP)"

2',3'-Dideoxyadenosine-5'-O-triphosphate (ddATP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire.

2',3'-Dideoxyadenosine-5'-Triphosphate (ddATP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination while PCR. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is widely useful in antiviral research and in a variety of biotechnology applications. ddATP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases.

Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.

Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977).

ddATP is an analogue of cyditine triphosphate (ATP) where both ribose hydroxy groups have been removed and replaced by hydrogen.

Specifications:
Purity: min. 95% (HPLC)
λmax = 259 nm; ε = 15000.01 λ x mmol-1 x cm-1
C10H16N5O11P3
MW = 475.2 g/mol (free acid)
Non-sterile 10mM sodium salt solution, pH7.6

Source:

synthetic

Classification:

CAS no.: 132619-65-9
eclass no.: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Spec. Product Description
General Data 1
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Accessory Items

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dNTP-Set (Na-Salz) - 100 mM Lösung

Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

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ddCTP

2',3'-Dideoxycytidine-5'-O-triphosphate (ddCTP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. 2',3'-Dideoxycytidine-5'-Triphosphate (ddCTP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination while PCR and is widely useful in antiviral research and in a variety of biotechnology applications. ddCTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977). ddCTP is an analogue of cyditine triphosphate (CTP) where both ribose hydroxy groups have been removed and replaced by hydrogen.

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ATP - Adenosine-5'-triphosphate

Adenosine triphosphate (ATP) is the universal and immediately available energy source in cells and an important regulator of energy providing processes. The molecule adenosine triphosphate consists of an adenine, the sugar ribose and three phosphate (α to γ) in ester (α) or anhydride bond (β and γ). It was shown that ATP has an inhibitory effect on the inflammatory response by abrogating secretion of TNF-α and promoting secretion of Interleukin-10.  Synonyms: ATP, Adenosin-5'-Triphosphat.  Also available from Genaxxon: NADH Na salt (reduced) >.

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dNTP Mix (Na salt) - 10mM

Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods. The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions. Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 10 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix withv2 mM dNTP mix , our dNTP set, or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

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