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ReproFast proofreading Polymerase

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Product information "ReproFast proofreading Polymerase"

ReproFast DNA Polymerase – Maximum Reliability for Your Standard PCRs


ReproFast DNA Polymerase – the robust all-round solution for efficient and precise amplification in your daily laboratory workflows. This innovative blend of classic Taq DNA polymerase and a proofreading polymerase in a finely tuned buffer delivers superior results across nearly all standard PCR applications – with minimal optimization effort.
ReproFast reliably handles standard PCRs up to 5 kb from human genomic DNA and up to 7 kb from lambda DNA with consistently high yield. Save time and reduce costs: uniform reaction conditions cover a broad range of template types, from routine assays to challenging targets.

The All-Round Polymerase – Key Advantages


  • Perfect balance: Taq for power and proofreading for accuracy – the ideal combination of speed, yield, and fidelity
  • Universally applicable: Optimized buffer for diverse standard PCR applications, even with varying templates
  • Proven performance: Up to 5 kb (human gDNA) / 7 kb (lambda DNA) – validated in routine and research workflows
  • Workflow boost: Fewer enzymes, less optimization, more results

Practical Benefits for the Laboratory


  • Streamlined PCR setups for cloning, genotyping, and screening
  • High reproducibility for service laboratories and high-throughput applications
  • A single enzyme reduces storage needs and workflow complexity – a smart choice for efficient lab work

Extended Options


Hot Start PCR: Available as Hot-Start version (ReproHot Proofreading Polymerase, M3012) : increased specificity, room-temperature setup, and immediate start without activation – ideal for sensitive reactions.

With ReproFast DNA Polymerase, you have a universal enzyme covering a wide range of applications – powerful, reliable, and efficient.

More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:

- M3002 Pwo Proofreading Polymerase
- M3004 Pfu Proofreading Polymerase
- M3012 ReproHot (KOD) Proofreading Polymerase
- AQ97 High Fidelity proofreading Polymerase

With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

Specifications:
5 U/µL
3' - 5' Exonuclease activity (proof-reading activity)

Application:

Polymerase mixture to be used for long fragments in PCR

Units:

One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C), 50 mM

Source:

recombinant

Classification:

eclass no.: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Safety Data Sheet
Protocols
Manuals
Category List

For every template/primer pair the optimal reaction conditions have to be evaluated empirically, changing the primer/template
ratio, the ionic strength (with MgSO4) and the cycle parameters (time and temperatures).

DNA Template

Amplification of templates with high GC content, strong secondary structure, low concentrations, or which produce products greater than 5 kb, may require adaptation of the following parameters:

  • Use high quality, purified DNA templates.
  • Approximately 10E4 copies of target DNA are required to detect product in 25-30 PCR cycles.
  • Use 1pg–1ng of plasmid or viral templates.
  • Use 1ng–1µg of genomic templates.
  • Higher DNA concentrations decrease amplicon specificity (i.e., extra bands are more likely), particularly when a large number of cycles are employed.
  • Use the higher DNA concentrations when fewer cycles are desired (e.g. to increase fidelity).

Primers

  • Generally 20-30 nucleotides in length.
  • Ideal GC content is 40-60%.
  • Space GC residues evenly within the primer.
  • Primer pairs should have Tms within 5°C of each other.
  • Avoid secondary structure (i.e., hairpins) within each primer and potential dimerization between the primers present.
  • When engineering sites into the end of primers, 4-6 extra bases should be added 5´ to the site.
  • Final concentration should be 0.05-1 µM, typically 0.1-0.5 µM of each primer.
  • Higher concentrations may increase secondary priming and create spurious amplification products.

Magnesium Concentration

  • 1.5-2.0 mM is optimal for Taq DNA Polymerase, but the ideal concentration depends on template, buffer, DNA and dNTPs (each has the potential to chelate magnesium).
  • If [Mg2+] is too low, no PCR product will be seen.
  • If [Mg2+] is too high, undesired PCR products may be seen.

Deoxyribonucleotide triphosphates (dNTPs) >

  • Typical concentration is 200 µM of each dNTP.
  • 50-100 µM enhances fidelity of polymerization, but reduces yields.
  • Higher concentrations increase yields particularly in long PCR, but can reduce fidelity.

DNA Polymerase

  • The choice of the correct polymerase depends among other things on the purpose as well as on the template used (standard PCR: Taq DNA Polymerase S > with high accuracy, Taq Polymerase E > with high yield; master mixes: Standard PCR master mix > or RedMasterMix > with red dye).
  • For multiplex PCR, there exist special multiplex master mixes >.
  • Hot start applications are recommended to increase specificity or if you use difficult templates. For this purpose there exist special Hot Start Polymerases >.
  • For PCRs for the purpose of cloning or other procedures requiring a low error rate, there are thermostable high fidelity proofing polymerases such as Pfunds >, ExactRun >, ReproFast >, or ReproHot (KOD) Proofreading Polymerase >. These enzymes make far fewer errors during amplification and increase the chances of an amplicon without mistakes.
  •  For genotyping and other applications where a high discrimination rate is required, there is a new highly selective DNA polymerase, SNP Pol DNA Polymerase >. It specifically distinguishes mismatched primer-template complexes and ony produces specific amplicons in case of perfectly matched primer pairs.
  • For the real-time quantitative PCR > there exist highly specialized qPCR master mixes. Hereby, the choice of the correct master mix depends on the qPCR device you are using. It is important to determine whether and how much ROX should be contained in the qPCR master mix and whether it should be a qPCR green master mix with green fluorescent dye > or a qPCR probe  master mix > without a fluorescent dye. A new lyophilized qPCR probe master mix in beads format, which is stable at room temperature, is available from Genaxxon (LyoBalls >).
  • The amount of DNA polymerase used in the PCR reaction can significantly influence the PCR result (use 1.25-1.5 units Taq Polymerase > for a 50μL volume).

General Guidelines

Annealing Temperature and Duration

  • Match the Tms within 5°C of each other.
  • Typical annealing temperatures are 5°C below the lowest primer's Tm and often fall in the range of 50-60°C.
  • Test higher annealing temperatures if spurious amplification products are observed.
  • Typical annealing times are 15-30 seconds.

Extension Time

  • Extensions are normally performed at 68°C.
  • In general, use extension times of one minute per 1000 base (1 kb) pairs (e.g. 3 minutes for a 3 kb product). 
  • For products less than 1 kb, use 45-60 seconds.
  • Products greater than 3 kb, or reactions using more than 30 cycles, may require longer extensions.

The amplification of templates with high GC content, strong secondary structures, low concentrations or amplicons more than 5 kb often require optimization of the PCR conditions. Typically, 15-30 seconds of denaturation should be performed at 95 ° C during PCR.

Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

Raising the bar: genus-specific nested PCR improves detection and lineage identification of avian haemosporidian parasites.
Sandrine Musa, Theo Hemberle, Staffan Bensch, Vaidas Palinauskas, Laima Baltrūnaitė, Friederike Woog, Ute Mackenstedt
Front Cell Infect Microbiol. 2024 Apr;14:1385599.
doi: 10.3389/fcimb.2024.1385599.
PMCID: PMC11089177.

Mitochondrial genome amplification of avian haemosporidian parasites from single-infected wildlife samples using a novel nested PCR approach. 
Sandrine Musa
Parasitol Res. 2023 Dec;122(12):2967-2975.
doi: 10.1007/s00436-023-07986-1.
PMCID: PMC10667411.

Melanocortin 4 receptor signaling and puberty onset regulation in Xiphophorus swordtails.
Ruiqi Liu, Kang Du, Jenny Ormanns, Mateus C Adolfi, Manfred Schartl
Gen Comp Endocrinol. 2020 Sep 1;295:113521.
doi: 10.1016/j.ygcen.2020.113521.
PMID: 32470471.

MpsAB is important for Staphylococcus aureus virulence and growth at atmospheric CO2 levels

Sook-Ha Fan, Patrick Ebner, Sebastian Reichert, Tobias Hertlein, Susanne Zabel, Aditya Kumar Lankapalli, Kay Nieselt, Knut Ohlsen, Friedrich Götz
Nat Commun. 2019; 10: 3627.
doi: 10.1038/s41467-019-11547-5
PMCID: PMC6689103

Industrial Acetogenic Biocatalysts: A Comparative Metabolic and Genomic Analysis

Frank R. Bengelsdorf, Anja Poehlein, Sonja Linder, Catarina Erz, Tim Hummel, Sabrina Hoffmeister, Rolf Daniel, Peter Dürre
Front Microbiol. 2016; 7: 1036. 
doi: 10.3389/fmicb.2016.01036
PMCID: PMC4935695

A Putative Non-Canonical Ras-Like GTPase from P. falciparum: Chemical Properties and Characterization of the Protein
Annette Kaiser, Barbara Langer, Jude Przyborski, David Kersting, Mirko Krüger
PLoS One. 2015; 10(11): e0140994. 
doi: 10.1371/journal.pone.0140994
PMCID: PMC4634863

Functionally redundant but dissimilar microbial communities within biogas reactors treating maize silage in co-fermentation with sugar beet silage
Susanne G Langer, Sharif Ahmed, Daniel Einfalt, Frank R Bengelsdorf, Marian Kazda
Microb Biotechnol. 2015 Sep; 8(5): 828–836. 
doi: 10.1111/1751-7915.12308
PMCID: PMC4554470

Analysis of the key enzymes of butyric and acetic acid fermentation in biogas reactors

Christina Gabris, Frank R Bengelsdorf, Peter Dürre
Microb Biotechnol. 2015 Sep; 8(5): 865–873. 
doi: 10.1111/1751-7915.12299
PMCID: PMC4554474

Genetic transformation of Knufia petricola A95 - a model organism for biofilm-material interactions

Steffi Noack-Schönmann, Tanja Bus, Ronald Banasiak, Nicole Knabe, William J Broughton, H Den Dulk-Ras, Paul JJ Hooykaas, Anna A Gorbushina
AMB Express. 2014; 4: 80. 
doi: 10.1186/s13568-014-0080-5
PMCID: PMC4230810

A Set of Engineered Escherichia coli Expression Strains for Selective Isotope and Reactivity Labeling of Amino Acid Side Chains and Flavin Cofactors
Jennifer Mehlhorn, Helena Steinocher, Sebastian Beck, John T. M. Kennis, Peter Hegemann, Tilo Mathes
PLoS One. 2013; 8(11): e79006. 
doi: 10.1371/journal.pone.0079006
PMCID: PMC3815312

Liver hyperplasia after tamoxifen induction of Myc in a transgenic medaka model

Luciana A. Menescal, Cornelia Schmidt, Daniel Liedtke, Manfred Schartl
Dis Model Mech. 2012 Jul; 5(4): 492–502. 
doi: 10.1242/dmm.008730
PMCID: PMC3380712

Gene Cluster Involved in the Biosynthesis of Griseobactin, a Catechol-Peptide Siderophore of Streptomyces sp. ATCC 700974
Silke I. Patzer, Volkmar Braun
J Bacteriol. 2010 Jan; 192(2): 426–435. 
doi: 10.1128/JB.01250-09
PMCID: PMC2805312

Replacement of a Phenylalanine by a Tyrosine in the Active Site Confers Fructose-6-phosphate Aldolase Activity to the Transaldolase of Escherichia coli and Human Origin
Sarah Schneider, Tatyana Sandalova, Gunter Schneider, Georg A. Sprenger, Anne K. Samland
J Biol Chem. 2008 Oct 31; 283(44): 30064–30072. 
doi: 10.1074/jbc.M803184200
PMCID: PMC2662071

Proofreading Polymerases and Master Mixes - Achieve Results Quickly and Accurately with GENAXXON

High Fidelity Proofreading DNA Polymerases offer high amplification rates combined with exceptional accuracy

Optimization of Annealing Temperature and other PCR Parameters

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Accessory Items - Zubehör

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Nuklease und DNA-freies Wasser für die PCR

Pure, quality tested, nuclease-free water is suitable for use in all experiments that require nuclease-free water, including molecular biology applications. Nuclease-free water is processed, to yield DNase, RNase, and nuclease-free, deionized water. Contamination of water with nucleases may lead to inconsitencies or even complete failures of experiments. For this Genaxxon offers different grades of nuclease free water: sterile, Diethylpyrocarbonate (DEPC) treated water (M6082 >) and nuclease free water (not DEPC treated) (M6340 >). Our Nuclease-Free Water is provided in nuclease-free PE bottles or in glass bottles.

Regular price: From €16.39
dNTP Mix (Na salt) - 10mM
Volume: 1 mL

Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods. The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions. Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 10 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix withv2 mM dNTP mix , our dNTP set, or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

Variants from €18.00
Regular price: €53.50
DNA Loading buffer I Fluoro (6x)

Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels.  Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel.  Please note: DNA Loading Buffer I Fluoro does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation (ca. 490nm). We therefore recommend using blue light to achieve the best possible results.

Regular price: From €25.00
Genaxxon's dNTP Mix 2mM Ready to use
Volume: 5 mL

Highly pure, HPLC purified dNTPs (>99%) delivered as a 2 mM solution of dATP, dCTP, dGTP and dTTP for use in qPCR, standard PCR, RT-PCR and Klenow reactions. Use 5 µL of Mix for PCR in 50µL reaction volume.The Genaxxon dNTP mix is optimized for its use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Mixture of dNTP sodium salt solutions (dATP, dCTP, dGTP und dTTP) Concentration: 2 mM of ach nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix with 10 mM dNTP mix, our dNTP set , or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

Variants from €25.00
Regular price: €70.00
Loading Dye with Bromophenol blue

Gel Loading Dye I 6X is a loading buffer for DNA application on gels. Buffer contains Glycerol, EDTA, Bromophenol Blue and Xylene cyanol. Genaxxon 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis.

Regular price: From €27.50
Loading Dye with Orange G

DNA 6X Loading buffer is a special loading buffer for DNA application on gels that enables viusalization of DNA bands smaller than 100 bp without problems. Buffer contains Glycerol, EDTA, Orange G. The special dye gives the opportunity to detect even DNA bands running below 65 bp. Other loading dye: M3308 and PCR dye: M3309.

Regular price: From €27.50
Coral Red PCR Buffer - coloured PCR buffer for visualization of pipetting steps

Coral Red Buffer is a 10-time dye solution as supplement for PCR buffers. This additive contains Glycerol, EDTA, a red and a yellow dye. 5µL of this solution are added to 45µL of the PCR assay before the PCR reaction to stain it red. An alternative to this product is the Genaxxon bioscience ready-to-use RedMastermix M3029. The RedMastermix contains all incredients necessary for PCR including a red dye that enables to control the different pipetting steps.

Regular price: From €29.50
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dNTP-Set (Na-Salz) - 100 mM Lösung
amount of substance: 4 x 100 µmol

Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

Variants from €33.25
Regular price: From €113.75
gm3094 100 bp dna ladder with additional 1.5kbp and 3.0kbp bands
Weight: 50 µg

Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker. The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL). The recommended amount of DNA marker per lane is 0,5μg (5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Regular price: €36.75
m3328 1kbp dna ladder with loading dye

The GenLadder 1kb (ready-to-use) 250bp - 10kbp-ladder is the ideal DNA size marker for fragment sizes from 250 - 10000bp. All bands light up to the same intensity except the 1000 bp and the 3000 bp band which are twice as bright for better orientation. Each band has been calibrated with respect to base pair size and DNA quantity. Thus, an accurate determination of product size and DNA concentration is possible. No ambiguous bands are visible. The GenLadder 250bp - 10kb can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. The DNA is already dissolved in buffer with 6X loading dye ready to use. Fragment sizes and amount of DNA per band: 10000bp ( 28ng/5µL), 8000bp (28ng/5µL), 6000bp (28ng/5µL), 5000bp (28ng/5µL), 4000bp ( 18ng/5µL), 2x3000bp (92ng/5µL), 2500bp (34ng/5µL), 2000bp (34ng/5µL), 1500bp (20ng/5µL), 2x1000bp (92ng/5µL), 750bp (23ng/5µL), 500bp (30ng/5µL), 250bp (45ng/5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Regular price: From €36.75
GenLadder 100 bp Plus with gel staining dye

The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL).Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Regular price: From €42.50
GenLadder XLarge up to 25 kbp - ready-to-use

With the synthetic GenLadder XLarge, even larger plasmid insertions and vector plasmids can be determined clearly and precisely in size by gel electrophoresis in the range of 250 - 25000 base pairs after restriction digests. The Genaxxon GenLadder XLarge shows 14 bands. The approximate mass of the DNA in each band is given for a 5µL load (0.52µg/lane). Additionally the 1000 bp and 3000 bp are intensified for better and faster orientation in 1.0 - 1.5% agarose gels. Please avoid repeated freez-thaw cycles. We recommend to portion the product into smaller aliquots. Source: PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH8.0) and 10mM EDTA. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Regular price: From €45.50
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Steriles DEPC-Wasser

Sterile distilled water free of DNAses, Rnases and Proteinases. Water treated with DEPC to ensure absence of Rnase. For that reason this water is especially suited for RT-PCR or other molecular biology application where 100% absence of RNAse is necessary (resuspension of DNA, dilution of enzymes, etc.). Genaxxon offers different grades of nuclease free water: sterile, Diethylpyrocarbonate (DEPC) treated water (M6082 >) and nuclease free water (not DEPC treated) (M6340 >).

Regular price: From €46.05
50bp DNA ladder - Ready-to-Use 50-1500 bp - Genmarker

The GenLadder 50bp (ready-to-use) is an ideal DNA size marker for fragment sizes from 50 - 1500bp. The bands at 200bp, 500bp and 1200bp bands light up more intensive for better orientation. Each band has been calibrated with respect to base pair size. Thus, an accurate determination of product size is possible. No ambiguous bands are visible. Band sizes: 50 (ca. 30ng/5µL), 100 (ca. 27ng/5µL), 150 (ca. 30ng/5µL), 200 (2x) (ca. 67ng/5µL), 250 (ca. 25ng/5µL), 300 (ca. 20ng/5µL), 350 (ca. 18ng/5µL), 400 (ca. 27ng/5µL), 450 (ca. 23ng/5µL), 500 (2x) (ca. 67ng/5µL), 600 (ca. 20ng/5µL), 700 (ca. 23ng/5µL), 800 (ca. 27ng/5µL), 900 (ca. 30ng/5µL), 1000 (ca. 33ng/5µL), 1200 (2X) (ca. 70ng/5µL) and 1500bp (ca. 40ng/5µL). GenLadder 50bp is already dissolved in loading buffer with tracking dye (Orange G) that does not overlab with bands in the range between 500bp and and smaller. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Regular price: From €46.50
dUTP solution 100mM

Deoxyuridine 5'-triphosphate, tetrasodium salt (dUTP) of purity >99% (HPLC). dUTP may be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. Product has been tested for PCR products of length up to 8kb (Taq DNA polymerase) and 0.8kb (Pfu DNA polymerase). The substitution of dTTP for dUTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme Uracil-DNA-Glycosylase, UDG >, can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Genaxxon offers also PCR dNTP-Mix 10 mM >, unser dNTP-Set as 4 x 100 mM solution > or modified nucleotides as Biotin-11-dUTP >. For your successful PCR you will find different enzymes or PCR master mixes: Taq DNA Polymerse (M3001) >, Proof-Reading Polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as our  ready-to-use RedMastermix (M3029) >, containing dNTPs already.

Regular price: From €48.00
Agarose LM - chemical structure

Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives gels with better properties and higher transparency than the standard normal melting point agarose. The agarose is equivalent to SeaPlaque™ from Lonza. Agarose LM is a low melting agarose with a very high separation capacity for large DNA fragments (>1000 bp), RNA and proteins. Enzymatic manipulations (eg, digestion, ligation, PCR, etc.) can be performed in the molten gel. Extraction of the DNA is therefore not necessary. Low melting Agarose LM is excellently suited for digestion with β-agarase (S5223). Large DNA fragments can be isolated very gently. The low melting agarose LM is used in the analytical and preparative field. For optimal results, the gel should be stored for at least 1 hour before use at +2°C to +8°C. Genaxxon offers agaroses for a wide variety of applications. There are agaroses with different melting points or with normal or high-resolution separation properties of DNA fragments. Standard melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (Standard Agarose LE (M3044 >)) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Regular price: From €50.40
Agarose LE - Standard Agarose
Weight: 100 g

Agarose LE is a standard agarose for the separation of DNA in the size range between 100bp and 25kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc. This agarose is comparable with, e.g. Agarose BioRagent, low EEO from Sigma or with the Universal-Agarose, peqGOLD from Peqlab. Free Taq DNA Polymerase test sample available! No shipping costs within Germany. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard normal melting agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 100bp up to 25kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Regular price: From €51.36
Low melting Agarose Tiny from Genaxxon - M3046

Agarose Tiny is a low-melting high resolution agarose that is suitable for molecular biology applications and produces gels with better separation properties and greater clarity than standard agarose. It is very transparent even at high gel concentrations (3.0 - 4.5%) and can be used with all buffer systems. The low melting temperature makes Agarose Tiny ideal for preparative DNA and RNA electrophoresis, while the low gelling temperature is ideal for tissue culture cell cloning experiments and viral plaque assays. In addition to the above mentioned properties, Agarose Tiny can also be used for high-resolution (2 bp) gel electrophoresis in the range from 10 to 500 bp. Example: PCR products or small DNA fragments after restriction digestion or in mutation analyses. Features:Like Lonza's SeaPlaque™, Agarose Tiny has a low melting point (<64°C, 3.0% gel) and gel point (<25°C, 3.0% gel) and low DNA-binding capacity. Their use is particularly recommended for preparative RNA and DNA gels. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.Recommended for the following applications:- RFLP (Restriction Fragment Length Polymorphism - AMPFLP (Amplified Fragment Length Polymorphism) - HVR (Hyper-Variable Regionen) - VNTR (Variable Numbers of Tandem Repeats) - STR (Short Tandem Repeats) - Tri und Tetra Nukleotid repeats

Regular price: From €56.72
Comparison of emission spectra safegel green stain and safegel red stain

SafeGel green stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel green stain is far more sensitive than EtBr without requiring a destaining step. SafeGel green stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel green stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.

Regular price: From €87.50
What customers say about SafeGel in comparison to EtBr

SafeGel red stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel red stain is far more sensitive than EtBr without requiring a destaining step. SafeGel red stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel red stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). Read in our blog why you should use Genaxxon's SafeGel now. For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.

Regular price: From €87.50
Agarose Tiny HT von Genaxxon

Our Agarose Tiny HT is a high resolution (+/-2 bp), normal melting agarose for fine resolution of small DNA fragments in the 20 bp to 1500 bp range. It can be used for genotyping, allele sizing and short tandem repeat analysis. Agarose Tiny works as a molecular screen and has twice the resolution of the finest sieving agaroses. It can discriminate between fragments which differ by only 2-4 bp in length and thus, is able to compete with acrylamide gels, being much easier to handle as the latter. Agarose Tiny HT 2% to 4% gels give similar results to Polyacrylamide 6% to 8% gels. For efficient resolution, the concentration of Agarose Tiny HT should be adjusted according to the range of band sizes analyzed, and the gel running buffer used (TAE or TBE). Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Regular price: From €91.68