Advantages at a glance
- 5 units/µL
- Storage buffer without glycerol.
- Supplied without buffer or MgCl2
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Product information "Glycerol free Taq DNA polymerase"
Glycerol free Taq DNA polymerase is developed for automation and freeze drying. It is a glycerol free formulation of the standard Ampliqon Taq DNA Polymerase and is well suited for automated routine PCR applications, or where accurate pipetting of small amounts is crucial.
Glycerol free Taq DNA polymerase is supplied without PCR buffer and without MgCl2.
Features:
- Glycerol free storage buffer
- Suitable for automated high throughput testing
- Suitable for robot-aided pipetting
- Suitable for applications involving freeze drying
- High product yield
- Processes up to 5 kb
- dUTP incorporation possible
Description:
Ampliqon Taq DNA Polymerase Glycerol Free exhibits both a 5'→3'dA DNA polymerase and a 5'→3' exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3'-5' exonuclease activity and proofreading ability.
Why glycerol?
Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.
Specifications:
5 units/µL
storage buffer without glycerol
Supplied without buffer or MgCl2
Units:
One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mMSource:
Thermus aquaticus strainClassification:
Documents:
Product descriptionCategory List
DNA Template
Amplification of templates with high GC content, strong secondary structure, low concentrations, or which produce products greater than 5 kb, may require adaptation of the following parameters:
- Use high quality, purified DNA templates.
- Approximately 10E4 copies of target DNA are required to detect product in 25-30 PCR cycles.
- Use 1pg–1ng of plasmid or viral templates.
- Use 1ng–1µg of genomic templates.
- Higher DNA concentrations decrease amplicon specificity (i.e., extra bands are more likely), particularly when a large number of cycles are employed.
- Use the higher DNA concentrations when fewer cycles are desired (e.g. to increase fidelity).
Primers
- Generally 20-30 nucleotides in length.
- Ideal GC content is 40-60%.
- Space GC residues evenly within the primer.
- Primer pairs should have Tms within 5°C of each other.
- Avoid secondary structure (i.e., hairpins) within each primer and potential dimerization between the primers present.
- When engineering sites into the end of primers, 4-6 extra bases should be added 5´ to the site.
- Final concentration should be 0.05-1 µM, typically 0.1-0.5 µM of each primer.
- Higher concentrations may increase secondary priming and create spurious amplification products.
Magnesium Concentration
- 1.5-2.0 mM is optimal for Taq DNA Polymerase, but the ideal concentration depends on template, buffer, DNA and dNTPs (each has the potential to chelate magnesium).
- If [Mg2+] is too low, no PCR product will be seen.
- If [Mg2+] is too high, undesired PCR products may be seen.
Deoxyribonucleotide triphosphates (dNTPs) >
- Typical concentration is 200 µM of each dNTP.
- 50-100 µM enhances fidelity of polymerization, but reduces yields.
- Higher concentrations increase yields particularly in long PCR, but can reduce fidelity.
DNA Polymerase
- The choice of the correct polymerase depends among other things on the purpose as well as on the template used (standard PCR: Taq DNA Polymerase S > with high accuracy, Taq Polymerase E > with high yield; master mixes: Standard PCR master mix > or RedMasterMix > with red dye).
- For multiplex PCR, there exist special multiplex master mixes >.
- Hot start applications are recommended to increase specificity or if you use difficult templates. For this purpose there exist special Hot Start Polymerases M3307 > or M3006 >.
- For PCRs for the purpose of cloning or other procedures requiring a low error rate, there are thermostable high fidelity proofing polymerases such as Pfu >, AQ97>, or ReproFast >. These enzymes make far fewer errors during amplification and increase the chances of an amplicon without mistakes.
- For genotyping and other applications where a high discrimination rate is required, there is a new highly selective DNA polymerase, SNP Pol DNA Polymerase >. It specifically distinguishes mismatched primer-template complexes and ony produces specific amplicons in case of perfectly matched primer pairs.
- For the real-time quantitative PCR there exist highly specialized qPCR master mixes >. Hereby, the choice of the correct master mix depends on the qPCR device you are using. Alternatively, you can simply use a universal master mix, such as the GNX Versa qPCR MasterMix 2X > from Genaxxon, which automatically ‘adjusts’ the correct amount of ROX for the respective instrument. Even if there are different PCR cyclers in the laboratory, you no longer need to worry about which master mix to use
- The amount of DNA polymerase used in the PCR reaction can significantly influence the PCR result (use 1.25-1.5 units Taq Polymerase > for a 50μL volume).
General Guidelines
Annealing Temperature and Duration
- Match the Tms within 5°C of each other.
- Typical annealing temperatures are 5°C below the lowest primer's Tm and often fall in the range of 50-60°C.
- Test higher annealing temperatures if spurious amplification products are observed.
- Typical annealing times are 15-30 seconds.
Extension Time
- Extensions are normally performed at 68°C.
- In general, use extension times of one minute per 1000 base (1 kb) pairs (e.g. 3 minutes for a 3 kb product).
- For products less than 1 kb, use 45-60 seconds.
- Products greater than 3 kb, or reactions using more than 30 cycles, may require longer extensions.
The amplification of templates with high GC content, strong secondary structures, low concentrations or amplicons more than 5 kb often require optimization of the PCR conditions. Typically, 15-30 seconds of denaturation should be performed at 95 ° C during PCR.
Accessory Items
Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives gels with better properties and higher transparency than the standard normal melting point agarose. The agarose is equivalent to SeaPlaque™ from Lonza. Agarose LM is a low melting agarose with a very high separation capacity for large DNA fragments (>1000 bp), RNA and proteins. Enzymatic manipulations (eg, digestion, ligation, PCR, etc.) can be performed in the molten gel. Extraction of the DNA is therefore not necessary. Low melting Agarose LM is excellently suited for digestion with β-agarase (S5223). Large DNA fragments can be isolated very gently. The low melting agarose LM is used in the analytical and preparative field. For optimal results, the gel should be stored for at least 1 hour before use at +2°C to +8°C. Genaxxon offers agaroses for a wide variety of applications. There are agaroses with different melting points or with normal or high-resolution separation properties of DNA fragments. Standard melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (Standard Agarose LE (M3044 >)) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods. The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions. Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 10 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix withv2 mM dNTP mix , our dNTP set, or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker. The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL). The recommended amount of DNA marker per lane is 0,5μg (5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Agarose LE is a standard agarose for the separation of DNA in the size range between 100bp and 25kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc. This agarose is comparable with, e.g. Agarose BioRagent, low EEO from Sigma or with the Universal-Agarose, peqGOLD from Peqlab. Free Taq DNA Polymerase test sample available! No shipping costs within Germany. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard normal melting agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 100bp up to 25kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
The GenLadder 1kb (ready-to-use) 250bp - 10kbp-ladder is the ideal DNA size marker for fragment sizes from 250 - 10000bp. All bands light up to the same intensity except the 1000 bp and the 3000 bp band which are twice as bright for better orientation. Each band has been calibrated with respect to base pair size and DNA quantity. Thus, an accurate determination of product size and DNA concentration is possible. No ambiguous bands are visible. The GenLadder 250bp - 10kb can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. The DNA is already dissolved in buffer with 6X loading dye ready to use. Fragment sizes and amount of DNA per band: 10000bp ( 28ng/5µL), 8000bp (28ng/5µL), 6000bp (28ng/5µL), 5000bp (28ng/5µL), 4000bp ( 18ng/5µL), 2x3000bp (92ng/5µL), 2500bp (34ng/5µL), 2000bp (34ng/5µL), 1500bp (20ng/5µL), 2x1000bp (92ng/5µL), 750bp (23ng/5µL), 500bp (30ng/5µL), 250bp (45ng/5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Accessory Items
ReproFast DNA Polymerase – Maximum Reliability for Your Standard PCRs ReproFast DNA Polymerase – the robust all-round solution for efficient and precise amplification in your daily laboratory workflows. This innovative blend of classic Taq DNA polymerase and a proofreading polymerase in a finely tuned buffer delivers superior results across nearly all standard PCR applications – with minimal optimization effort. ReproFast reliably handles standard PCRs up to 5 kb from human genomic DNA and up to 7 kb from lambda DNA with consistently high yield. Save time and reduce costs: uniform reaction conditions cover a broad range of template types, from routine assays to challenging targets. The All-Round Polymerase – Key Advantages Perfect balance: Taq for power and proofreading for accuracy – the ideal combination of speed, yield, and fidelity Universally applicable: Optimized buffer for diverse standard PCR applications, even with varying templates Proven performance: Up to 5 kb (human gDNA) / 7 kb (lambda DNA) – validated in routine and research workflows Workflow boost: Fewer enzymes, less optimization, more results Practical Benefits for the Laboratory Streamlined PCR setups for cloning, genotyping, and screening High reproducibility for service laboratories and high-throughput applications A single enzyme reduces storage needs and workflow complexity – a smart choice for efficient lab work Extended Options Hot Start PCR: Available as Hot-Start version (ReproHot Proofreading Polymerase, M3012) : increased specificity, room-temperature setup, and immediate start without activation – ideal for sensitive reactions. With ReproFast DNA Polymerase, you have a universal enzyme covering a wide range of applications – powerful, reliable, and efficient. More High-Fidelity Proofreading Polymerases from Genaxxon bioscience: - M3002 Pwo Proofreading Polymerase- M3004 Pfu Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase- AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.
High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-E'. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer contains 25mM MgCl2. Taq DNA Polymerase test sample available! No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
RedMasterMix (2x) - PCR MasterMix with red dye to help visualize pipetting and mixing steps. Electrophoresis can be performed immediately after PCR without the need of a gel loading buffer. This makes this MasterMix time efficient, cost efficient and a reliable choice for the best PCR results with high efficiency. One tube, one pipetting step. RedMasterMix (2x) is delivered to you as a optimized, ready-to-use solution containing- Taq DNA Polymerase - dNTPs- MgCl2- a red dye- reaction bufferfor efficient amplification of DNA. Additionally the RedMasterMix (2x) contains an additive and a red dye for proceeding with electrophoresis after PCR without adding loading buffer. Just add your primers and template DNA. This PCR MasterMix (2x) has been optimized for use in routine PCR amplification of DNA templates in the range of 0.2-4kb. The special formulation of our PCR RedMasterMix (2x) can withstand repeated freezing/thawing without compromising yields, sensitivity or results, when used as directed. The PCR MasterMix with red dye is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for at least 24 months.Our RedMastermix can also be used for Sanger sequencing. Just dilute the PCR reaction 1:8 after PCR, or purify using spin columns and then apply. Read now in our blog how Genaxxon's RedMasterMix can also simplify your laboratory work. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.
The High-Fidelity Pwo proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pwo DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus woesei. Pwo proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase. A mixture of Taq DNA Polymerase and Pwo DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermo stability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:- M3003 ReproFast Proofreading Polymerase- M3004 Pfu Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase - AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.
The High-Fidelity Pfu proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pfu DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus furiosus (Pfu). Pfu proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase. A mixture of Taq DNA Polymerase and Pfu DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermo stability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:- M3003 ReproFast Proofreading Polymerase- M3002 Pwo Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase - AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.
For reliable and easy PCR: PCR Master Mix for standard PCR applications (e.g. screenings) for amplifying DNA fragments <5kb. It is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by routine PCR (one tube, one pipetting step). Just add your primers and template DNA - that´s all. The special formulation of our PCR Master Mix guarantees you reproducible results even after repeated freezing/thawing steps leading to good yields and sensitivity. The PCR Master Mix is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for 24 months. Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.