Advantages at a glance
- Glycerol free - lyo-ready
- 200 units/µL
- Inhibits common RNases (RNase A, RNase B, RNase C and human placental RNase)
- Does not inhibit RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H.
- Compatible with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases.
- Tested for the absence of DNases and RNases
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Product information "Glycrol free RNase Inhibitor (RNAsin)"
- RT-PCR
- cDNA synthesis
- In vitro transcription/translation
- Enzymatic RNA labelling reactions
- In vitro virus detection.
- Improvement of RNA translation in homologous systems.
- Preparations of RNase-free antibodies.
- Any application where eukaryotic RNase contamination is a potential problem.
Advantages:
200 units/µL
Recombinant protein of murine origin
Inhibits common eukaryotic RNases (RNase A, RNase B, RNase C and human placental RNase)
Does not inhibit RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H.
Compatible with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases.
Active over a broad pH range (pH 5-8).
Tested for the absence of DNases and RNases
Application:
- Reversible inhibition of RNase- Suitable for all applications with RNA, especially for reverse transcription of RNA into cDNA- Useful if eukaryotic RNase contamination occursUnits:
one unit of RNase inhibitor is defined as the amount of protein required to inhibit 50% of the activity of 5ng of RNase ASource:
E. coli strain that carries the murine Ribonuclease Inhibitor geneClassification:
Documents:
Safety Data SheetProduct description
Accessory Items - Zubehör
Agarose LE is a standard agarose for the separation of DNA in the size range between 100bp and 25kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc. This agarose is comparable with, e.g. Agarose BioRagent, low EEO from Sigma or with the Universal-Agarose, peqGOLD from Peqlab. Free Taq DNA Polymerase test sample available! No shipping costs within Germany. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard normal melting agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 100bp up to 25kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives gels with better properties and higher transparency than the standard normal melting point agarose. The agarose is equivalent to SeaPlaque™ from Lonza. Agarose LM is a low melting agarose with a very high separation capacity for large DNA fragments (>1000 bp), RNA and proteins. Enzymatic manipulations (eg, digestion, ligation, PCR, etc.) can be performed in the molten gel. Extraction of the DNA is therefore not necessary. Low melting Agarose LM is excellently suited for digestion with β-agarase (S5223). Large DNA fragments can be isolated very gently. The low melting agarose LM is used in the analytical and preparative field. For optimal results, the gel should be stored for at least 1 hour before use at +2°C to +8°C. Genaxxon offers agaroses for a wide variety of applications. There are agaroses with different melting points or with normal or high-resolution separation properties of DNA fragments. Standard melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (Standard Agarose LE (M3044 >)) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
Our Agarose Tiny HT is a high resolution (+/-2 bp), normal melting agarose for fine resolution of small DNA fragments in the 20 bp to 1500 bp range. It can be used for genotyping, allele sizing and short tandem repeat analysis. Agarose Tiny works as a molecular screen and has twice the resolution of the finest sieving agaroses. It can discriminate between fragments which differ by only 2-4 bp in length and thus, is able to compete with acrylamide gels, being much easier to handle as the latter. Agarose Tiny HT 2% to 4% gels give similar results to Polyacrylamide 6% to 8% gels. For efficient resolution, the concentration of Agarose Tiny HT should be adjusted according to the range of band sizes analyzed, and the gel running buffer used (TAE or TBE). Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
Coral Red Buffer is a 10-time dye solution as supplement for PCR buffers. This additive contains Glycerol, EDTA, a red and a yellow dye. 5µL of this solution are added to 45µL of the PCR assay before the PCR reaction to stain it red. An alternative to this product is the Genaxxon bioscience ready-to-use RedMastermix M3029. The RedMastermix contains all incredients necessary for PCR including a red dye that enables to control the different pipetting steps.
Gel Loading Dye I 6X is a loading buffer for DNA application on gels. Buffer contains Glycerol, EDTA, Bromophenol Blue and Xylene cyanol. Genaxxon 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis.
Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels. Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel. Please note: DNA Loading Buffer I Fluoro does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation (ca. 490nm). We therefore recommend using blue light to achieve the best possible results.
DNA 6X Loading buffer is a special loading buffer for DNA application on gels that enables viusalization of DNA bands smaller than 100 bp without problems. Buffer contains Glycerol, EDTA, Orange G. The special dye gives the opportunity to detect even DNA bands running below 65 bp. Other loading dye: M3308 and PCR dye: M3309.
Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods. The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions. Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 10 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix withv2 mM dNTP mix , our dNTP set, or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
Highly pure, HPLC purified dNTPs (>99%) delivered as a 2 mM solution of dATP, dCTP, dGTP and dTTP for use in qPCR, standard PCR, RT-PCR and Klenow reactions. Use 5 µL of Mix for PCR in 50µL reaction volume.The Genaxxon dNTP mix is optimized for its use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Mixture of dNTP sodium salt solutions (dATP, dCTP, dGTP und dTTP) Concentration: 2 mM of ach nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix with 10 mM dNTP mix, our dNTP set , or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL).Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker. The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL). The recommended amount of DNA marker per lane is 0,5μg (5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
The GenLadder 1kb (ready-to-use) 250bp - 10kbp-ladder is the ideal DNA size marker for fragment sizes from 250 - 10000bp. All bands light up to the same intensity except the 1000 bp and the 3000 bp band which are twice as bright for better orientation. Each band has been calibrated with respect to base pair size and DNA quantity. Thus, an accurate determination of product size and DNA concentration is possible. No ambiguous bands are visible. The GenLadder 250bp - 10kb can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. The DNA is already dissolved in buffer with 6X loading dye ready to use. Fragment sizes and amount of DNA per band: 10000bp ( 28ng/5µL), 8000bp (28ng/5µL), 6000bp (28ng/5µL), 5000bp (28ng/5µL), 4000bp ( 18ng/5µL), 2x3000bp (92ng/5µL), 2500bp (34ng/5µL), 2000bp (34ng/5µL), 1500bp (20ng/5µL), 2x1000bp (92ng/5µL), 750bp (23ng/5µL), 500bp (30ng/5µL), 250bp (45ng/5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
The GenLadder 50bp (ready-to-use) is an ideal DNA size marker for fragment sizes from 50 - 1500bp. The bands at 200bp, 500bp and 1200bp bands light up more intensive for better orientation. Each band has been calibrated with respect to base pair size. Thus, an accurate determination of product size is possible. No ambiguous bands are visible. Band sizes: 50 (ca. 30ng/5µL), 100 (ca. 27ng/5µL), 150 (ca. 30ng/5µL), 200 (2x) (ca. 67ng/5µL), 250 (ca. 25ng/5µL), 300 (ca. 20ng/5µL), 350 (ca. 18ng/5µL), 400 (ca. 27ng/5µL), 450 (ca. 23ng/5µL), 500 (2x) (ca. 67ng/5µL), 600 (ca. 20ng/5µL), 700 (ca. 23ng/5µL), 800 (ca. 27ng/5µL), 900 (ca. 30ng/5µL), 1000 (ca. 33ng/5µL), 1200 (2X) (ca. 70ng/5µL) and 1500bp (ca. 40ng/5µL). GenLadder 50bp is already dissolved in loading buffer with tracking dye (Orange G) that does not overlab with bands in the range between 500bp and and smaller. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
With the synthetic GenLadder XLarge, even larger plasmid insertions and vector plasmids can be determined clearly and precisely in size by gel electrophoresis in the range of 250 - 25000 base pairs after restriction digests. The Genaxxon GenLadder XLarge shows 14 bands. The approximate mass of the DNA in each band is given for a 5µL load (0.52µg/lane). Additionally the 1000 bp and 3000 bp are intensified for better and faster orientation in 1.0 - 1.5% agarose gels. Please avoid repeated freez-thaw cycles. We recommend to portion the product into smaller aliquots. Source: PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH8.0) and 10mM EDTA. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Pure, quality tested, nuclease-free water is suitable for use in all experiments that require nuclease-free water, including molecular biology applications. Nuclease-free water is processed, to yield DNase, RNase, and nuclease-free, deionized water. Contamination of water with nucleases may lead to inconsitencies or even complete failures of experiments. For this Genaxxon offers different grades of nuclease free water: sterile, Diethylpyrocarbonate (DEPC) treated water (M6082 >) and nuclease free water (not DEPC treated) (M6340 >). Our Nuclease-Free Water is provided in nuclease-free PE bottles or in glass bottles.
SafeGel green stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel green stain is far more sensitive than EtBr without requiring a destaining step. SafeGel green stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel green stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.
SafeGel red stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel red stain is far more sensitive than EtBr without requiring a destaining step. SafeGel red stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel red stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). Read in our blog why you should use Genaxxon's SafeGel now. For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.
Sterile distilled water free of DNAses, Rnases and Proteinases. Water treated with DEPC to ensure absence of Rnase. For that reason this water is especially suited for RT-PCR or other molecular biology application where 100% absence of RNAse is necessary (resuspension of DNA, dilution of enzymes, etc.). Genaxxon offers different grades of nuclease free water: sterile, Diethylpyrocarbonate (DEPC) treated water (M6082 >) and nuclease free water (not DEPC treated) (M6340 >).
Accessory Items - related products
The Genaxxon RNase Inhibitor is a recombinant (E.coli) murine protein which inhibits ribonucleases (RNases) A, B and C. It does not inhibit RNase 1, RNase H, RNase T1, S1 Nuclease. Native RNase-Inhibitor from murine placenta exerts its inhibitory effect by binding non-covalently to RNases in a 1:1 ratio with high affinity. There is no inhibition of polymerase activity when this RNase Inhibitor is used with AMV Reverse Transcriptase, M-MuLV Reverse Transcriptase, Taq DNA Polymerase or Phage RNA Polymerase (SP6, T7, or T3).The enzyme is active over a broad pH range between 5 and 9, with a maximum activity at pH7 – pH8. The inhibition of RNases is a reversible process. Reagents as urea or thiol reagents will lead to the dissociation of the Inhibitor-RNase-complex, resulting in re-natured and functional RNase and irreversibly deactivated RNase inhibitor.Applications: RT-PCRcDNA synthesisIn vitro transcription/translationEnzymatic RNA labelling reactionsIn vitro virus detection. Improvement of RNA translation in homologous systems.Preparations of RNase-free antibodies. Any application where eukaryotic RNase contamination is a potential problem. Advantages: The recombinant murine RNase inhibitor does not contain the cysteine pair that was identified to cause the high sensitivity against oxidation resulting in the inactivation of the human RNase inhibitor (1). As a result, the murine RNase inhibitor has significantly improved oxidation resistance compared to the human and porcine RNase inhibitors. In addition, murine RNase inhibitor is much more stable at low low DTT concentrations (less than 1 mM), what makes it ideal for reactions where high DTT concentrations interfere with the reaction (e.g. real-time RT-PCR). Product does not contain glycerol => lyo-ready product (ready for preparations of freeze-dried products).Recommendations: Since the RNase inhibitor protein is sensitive to denaturing agents and conditions, whereas RNases are not, care must be taken to avoid high concentrations of urea or other denaturing agents and temperatures above 50-55°C. The recommended concentration of the RNase inhibitor is 1 unit/µL. During set-up of a reaction, RNase inhibitor should be added before other components are added (enzymes, plasmids from mini preps, etc.).References1. Kim, B.M. et al. Protein Science. 8, 430-434.
The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is an RNA-dependent DNA polymerase lacking the RNase H activity. The enzyme synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. M-MuLV RT will also extend primers hybridized to single-stranded DNA! The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase. Genaxxon offers the HotScriptase master mixes (M3062 >; M3064 >) with which it is possible to synthesize double stranded DNA completely without an isothermal transcription step. Time saving: up to 50%! No need for Superscript!Money saving: high! With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
Oligo (dT)20 are single stranded oligodeoxyribonucleotides containing only deoxythymine (dT) to be able to prime with the poly(A) tail of mRNA molecules. Primers are quality controlled (MS), purified (reversed phase HPLC), aliquoted and lyophilized and came with a guaranteed amount of at least 5OD (about 27.2nmol (163.7µg)). Oligo (dT)20 Primer is designed to initiate the synthesis of cDNA from total RNA in a reverse transcription reaction, where Reverse Transcriptase, e.g. MMuLV (M3042) > is starting the reaction from the poly-A-end of mRNA. It can also be used for generation of labeled cDNA to screen microarrays. A mixture (1:1 ratio) of random hexamer primers (M3038 >) and Oligo(dT)20 primer may improve the sensitivity of cDNA synthesis as it will especially improve the efficiency of transcripts longer than 600 bp. Guaranteed delivery of at least 5OD (equals about 27.2nmol (163.7µg)). Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted. Dissolve content (5 OD) in 271.5μL of molecular biology grade water for an end concentration of 100 pmol/μL (100μM). 760μL (100μM) are sufficient for 760 reverse transcriptions of 20μL each. Oligo (dT)20 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase, such as MMuLV or AMV. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)20 ensures that the 3´ end of mRNAs are represented. Oligo(dT)20 Primer may be better suited for the new generation of Reverse Transcriptases like SuperScript™ that work at higher temperatures as the longer Oligo(dT)20 Primer enable annealing in reverse transcription reactions at higher temperatures. Applications• cDNA synthesis from total RNA in a reverse transcription reaction.• optimal choice for construction of cDNA libraries from eukaryotic mRNAs.• Full length cDNA cloning• 3’ rapid amplification of cDNA ends (3’ RACE)• Generation of labelled cDNA for screening of microarrays.Oligo (dT)20 Primer can not be used together with degraded RNA, prokaryotic RNA or miRNA (lack of poly(A) tail). Primer Sequence: 5´ – d (TTT TTT TTT TTT TTT TTT TT) –3´
Ready to use One-Step RT-PCR MasterMix - Standard mix. Our RT-PCR One-Step Kit is designed for performing highly sensitive and specific RT-PCR. The kit is based on a genetically engineered reverse transcriptase with enhanced thermal stability resulting in an increased specificity, high cDNA yield and an improved efficiency for highly structured and long cDNA fragments. The kits contains all reagents required for RT-PCR (except template and primer) in one kit combining simple handling with high flexibility. The premium quality polymerases, ultrapure dNTPs and the optimized complete reaction buffers ensure superior amplification results. RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.In one-step RT-PCR all components of reverse transcription (RT) and PCR are mixed in one tube prior to starting the reaction and thus carried out sequentially without opening the tube. This offers tremendous convenience when applied to analysis of single targets from multiple samples of RNA and minimizes the risk of contaminations.Genaxxon offers also a “Zero-Step” HotScriptase RT (M3056) a RNA/DNA Polymerase that enables direct PCR from RNA without an isothermal reverse transcription step. Just starting the PCR protocol with specific primers. Especially suited for RNA targets that exhibit a high degree of secondary structure, for self- or cross-complementary primers. The Challenger Hotstart RT-qPCR 4x Mastermix (M3060 >) is particularly suitable for RNA that exhibits a high degree of secondary structures or for complementary primers!
Our One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA templates using SybrGreen®. The ready-to-use mixture is based on a genetically modified reverse transcriptase with improved thermal stability, which offers increased specificity, a high cDNA yield and improved efficiency for highly structured and long cDNA fragments.The 2X Mastermix contains all the reagents required for the RT-qPCR (except for the template and primer) to ensure quick and easy handling at a minimum of pipetting steps. The high-quality enzymes and the optimized reaction buffer with high-purity dNTPs guarantee superior real-time PCR results.This Master Mix does not contain ROX - please make sure to choose the right setting on your qPCR cycler. A list of common thermal cyclers and their ROX compatibility can be found under the "Tips" tab.RT-qPCR is used to amplify double-stranded DNA from single-stranded RNA templates to enable rapid real-time quantification of RNA targets. In the reverse transcription step, the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA). The cDNA molecules are amplified by the HotStart DNA polymerase. The HotStart polymerase activity is blocked at ambient temperature and will be activated at the beginning of the initial denaturation. This thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during the PCR setup.One-Step RT-qPCR offers enormous convenience when analyzing targets from multiple RNA samples and minimizes the risk of contamination. The Challenger Hotstart RT-qPCR 4x Mastermix (M3060 >) is particularly suitable for RNA that exhibits a high degree of secondary structures or for complementary primers! With our high-quality dNTPs, available as a set (M3015) or as a mix (M3016), as well as our DNA markers and affordable standard agarose, we offer you additional products for PCR.Time saving: up to 50%! No need for Superscript!Money saving: high!
Our One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA templates using probe-based assays such as Taqman®, Beacons, MGB or Mediator Probes and can be directly used with RNA as a starting material. The ready-to-use mixture is based on a genetically modified reverse transcriptase with improved thermal stability, which offers increased specificity, a high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The 2X Mastermix contains all the reagents required for the RT-qPCR (except for the template, primer and probes) to ensure quick and easy handling at a minimum of pipetting steps. The high-quality enzymes and the optimized reaction buffer with high-purity dNTPs guarantee superior real-time PCR results. The sophisticated buffer system provides fast kinetics, a RNA sensitivity of <10 fg and target amplification even for difficult templates and multiplexing applications for more than 6 targets.This Master Mix does not contain ROX - please make sure to choose the right setting on your qPCR cycler. A list of common thermal cyclers and their ROX compatibility can be found under the "Tips" tab.RT-qPCR is used to amplify double-stranded DNA from single-stranded RNA templates to enable rapid real-time quantification of RNA targets. In the reverse transcription step, the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA). The cDNA molecules are amplified by the HotStart DNA polymerase. The HotStart polymerase activity is blocked at ambient temperature and will be activated at the beginning of the initial denaturation. This thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during the PCR setup. One-Step RT-qPCR offers enormous convenience when analyzing targets from multiple RNA samples and minimizes the risk of contamination. The Challenger Hotstart RT-qPCR 4x Mastermix (M3060 >) is particularly suitable for RNA that exhibits a high degree of secondary structures or for complementary primers!With our high-quality dNTPs, available as a set (M3015) or as a mix (M3016), as well as our DNA markers and affordable standard agarose, we offer you additional products for PCR.Time saving: up to 50%! No need for Superscript!Money saving: high!
Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6]. Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted and came with a guaranteed amount of at least 5OD (about 76.1nmol (136.4µg)). Hexanucleotide primers are a mixture of random 5'-hydroxyl hexanucleotides or hexamers, and can be used to quickly and efficiently prepare radioactive or non-radioactive probes using a DNA polymerase and a suitable DNA template or for cDNA synthesis from mRNA. The heterogeneous nature of the random primers ensures that all possible sequences will be represented in the probe mixture. As a result, these primers are well suited for generating as long as possible (complete) transcripts of the RNA, transcribing the 5 'ends, in particular for long RNA templates. As an alternative you can also use Oligo(dT)-Primer > for reverse transcription. Oligo(dT) primer bind to the polyA tail of RNA and therefore transcription will start always at the 3'-end of the RNA. The guaranteed delivery quantity of 5OD, corresponding to approx. 76.1nmol, or 136.4μg, can be dissolved in 760μL DEPC water to give a concentration of 100pmol/μL (100μM), or approx. 180μg/μL. If using between 50ng and 250ng of the hexamers per 20μL reverse transcription reaction, 760μL (100μM) will be sufficient for 460 to 2280 reverse transcriptase reactions.